White-tailed deer S96 prion protein does not support stable in vitro propagation of most common CWD

Discussion in 'Wildlife Diseases' started by flounder, May 31, 2021.

  1. Published: 27 May 2021

    White-tailed deer S96 prion protein does not support stable in vitro propagation of most common CWD strains

    Alicia Otero, Camilo Duque Velásquez, Judd Aiken & Debbie McKenzie

    https://www.nature.com/articles/s41598-021-90606-8

    better hurry up in Texas...sad...terry

    SUNDAY, MAY 23, 2021

    TEXAS 267 DIFFERENT SITES HAVE RECEIVED DEER FROM AT LEAST ONE OF THE TWO RECENT CWD POSITIVE FACILITIES

    https://chronic-wasting-disease.blogspot.com/2021/05/texas-267-different-sites-have-received.html

    https://transmissiblespongiformence.../texas-267-different-sites-have-received.html

    TEXAS CWD STRAIN

    77. Assessing chronic wasting disease strain differences in free-ranging cervids across the United States

    ABSTRACT

    snip...

    Results: Our results have found significant differences in glycoform ratio between CWD from Michigan and Colorado, but no differences were observed in conformational stability assays. Interestingly, when testing our CWD isolates from Texas to analyse electrophoretic mobility and glycoform ratio, we found that these samples did not exhibit the characteristic band shift when treated with PK, but PK resistant material remained. Additionally, results from our conformational stability assay demonstrate a unique profile of these Texas isolates. Testing of samples from Missouri is currently underway.

    Conclusions: Thus far, our data indicate that there are strain differences between CWD circulating in Michigan and CWD in Colorado and provide important insight into CWD strain differences between two non-contiguous outbreaks. We have also identified a unique strain of CWD in Texas with biochemical strain properties not seen in any of our other CWD isolates. These results highlight the importance of continued surveillance to better understand this devastating disease. These results have important implications for CWD emergence, evolution and our understanding of prion strain heterogeneity on the landscape.

    https://www.tandfonline.com/doi/full/10.1080/19336896.2019.1615197

    One day in late February, in their laboratory in Fort Collins, Colorado, Wagner and Zabel compared the prions from the brains of CWD-infected deer in Texas with those of elk in Colorado. They want to know if the proteins were all mangled in the same way, or not. “If they are different, this would suggest that we have different strain properties, which is evidence as we're building our case that we might have multiple strains of CWD circulating in the U.S.,” says Wagner.

    Step one is to see if they’re equally easy to destroy using a chemical called guanidine. The shape of a prion dictates everything, including the way it interacts with an animal’s cells and the ease with which chemicals can unfold it.

    “Moment of truth,” said Wagner, as she and Zabel huddled around a computer, waiting for results to come through. When they did, Zabel was surprised.

    “Wow,” he said. “Unlike anything we've seen before.”

    The prions from the Texas deer were a lot harder to destroy than the ones from the Colorado elk. In fact, the guanidine barely damaged them at all. “We’ve never seen that before in any prion strain, which means that it has a completely different structure than we've ever seen before,” says Zabel. And that suggests that it might be a very different kind of chronic wasting disease. The researchers ran the same test on another Texas deer, with the same results.

    https://www.hcn.org/articles/wildli...-herds-may-also-threaten-human-health-science

    TUESDAY, MAY 11, 2021

    A Unique Presentation of Creutzfeldt-Jakob Disease in a Patient Consuming Deer Antler Velvet

    https://creutzfeldt-jakob-disease.blogspot.com/2021/05/a-unique-presentation-of-creutzfeldt.html

    Saturday, May 1, 2021

    ***> Clinical Use of Improved Diagnostic Testing for Detection of Prion Disease <***

    https://prionprp.blogspot.com/2021/05/clinical-use-of-improved-diagnostic.html

    kind regards, terry